Plasmid

Part:BBa_K3600012:Design

Designed by: yasmine koubaa   Group: iGEM20_EPFL   (2020-10-27)


Lys2-GFP dropout multi-gene backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 1309
    Illegal XbaI site found at 1009
    Illegal XbaI site found at 1318
    Illegal SpeI site found at 2687
    Illegal PstI site found at 2696
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 1309
    Illegal SpeI site found at 2687
    Illegal PstI site found at 2696
    Illegal NotI site found at 793
    Illegal NotI site found at 3251
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2723
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 1309
    Illegal BglII site found at 2711
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 1309
    Illegal XbaI site found at 1009
    Illegal XbaI site found at 1318
    Illegal SpeI site found at 2687
    Illegal PstI site found at 2696
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 1309
    Illegal XbaI site found at 1009
    Illegal XbaI site found at 1318
    Illegal SpeI site found at 2687
    Illegal PstI site found at 2696
    Illegal NgoMIV site found at 2612
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2501
    Illegal BsaI.rc site found at 1483
    Illegal SapI.rc site found at 826
    Illegal SapI.rc site found at 1667


Design Notes

.


Source

.

References